Antimicrobial activity of Boscia Senegalensis plant extracts against some bacterial strains in water treatment
DOI:
https://doi.org/10.54388/jkues.v4i1.307Keywords:
Antibacterial activity, Boscia Senegalensis, bark and leave Extracts, Bacteria.Abstract
AbstractThe study aimed to explore the possibility of utilizing the efficiency of these natural extracts in purifying and sterilizing drinking water, to improve water quality instead of other chemicals, by evaluating the in vitro activity of the plant Boscia senegalensis (Pers.) and comparing the differences between the use of leaf and bark extracts against some pathogenic bacteria strains found in the drinking water of East and Canter of CHAD. To achieve this, ethanolic extracts were used against some types of bacteria, including gram-negative such as Salmonella typhi (SL), Escherichia coli (EC), and Klebsiella pneumoniea (KL). The extracts showed activity with an inhibition zone for leave and bark extracts of about (18, 24, 21), (26, 21, and 23) mm against SL, EC, and KL, respectively. The “MCoI” the minimum concentration of inhibitory, and ”MCoB” the minimum concentration of bacteriocidal, the values were (6.25 and 12.50) mg per ml, respectively. Antibacterial activity was evaluated by measuring the inhibition zone area, after, the results were compared with a standard antibiotic (ciprofloxacin). All leaf and bark extracts showed antibacterial and anti fungal activity. However, bark extracts showed more advantages, possibly due to the presence of natural substances as secondary metabolites in the plant.The study aimed to explore the possibility of utilizing the efficiency of these natural extracts in purifying and sterilizing drinking water, to improve water quality instead of other chemicals, by evaluating the in vitro activity of the plant Boscia Senegalensis (Pers.) and comparing the differences between the use of leaf and bark extracts against some pathogenic bacteria strains found in the drinking water of East and Center of CHAD. To achieve this, ethanolic extracts were used against three bacterial strains including Gram-negative such as Salmonella typhi (SL), Escherichia coli (EC), and Klebsiella pneumoniea (KL). The extracts showed activity with an inhibition zone for leaf and bark extracts of about (18, 24, 21), (26, 21, and 23) mm against SL, EC, and KL, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were (6.25 and 12.5) mg/ml, respectively. Antibacterial activity was evaluated by measuring the diameter of the inhibition zone, and results were compared with a standard antibiotic (Ciprofloxacin). All leaf and bark extracts showed antibacterial and anti-fungal activity. However, bark extracts showed more advantages, possibly due to the presence of secondary metabolites in the plant.
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